Spatial fluorescence cross-correlation spectroscopy by means of a spatial light modulator
نویسندگان
چکیده
منابع مشابه
Spatial fluorescence cross-correlation spectroscopy by means of a spatial light modulator.
Spatial fluorescence cross-correlation spectroscopy is a rarely investigated version of fluorescence correlation spectroscopy, in which the fluorescence signals from different observation volumes are cross-correlated. In the reported experiments, two observation volumes, typically shifted by a few microm, are produced, with a spatial light modulator and two adjustable pinholes. We illustrated t...
متن کاملAnalysis of spatial light modulator contrast ratios and optical correlation.
We have performed a general analysis of optical correlators with spatal light modulators (SLM's) whose primary defect is a finite contrast ratio (CR). Our mathematical analysis identifies three noise terms that appear in addition to the correlation term. The filter SLM contains either a phase-only filter (POF) or a binary-phase-only filter (BPOF). Insertion of a dc block at the center of the fi...
متن کاملCorrelation with a spatial light modulator having phase and amplitude cross coupling.
In correlation filtering a spatial light modulator is traditionally modeled as affecting only the phase or only the amplitude of light. Usually, however, a single operating parameter affects both phase and amplitude. An integral constraint is developed that is a necessary condition for optimizing a correlation filter having single parameter coupling between phase and amplitude. The phase-only f...
متن کاملQuantitative phase microscopy using defocusing by means of a spatial light modulator.
A new method for recovery the quantitative phase information of microscopic samples is presented. It is based on a spatial light modulator (SLM) and digital image processing as key elements to extract the sample's phase distribution. By displaying a set of lenses with different focal power, the SLM produces a set of defocused images of the input sample at the CCD plane. Such recorded images are...
متن کاملFluorescence correlation spectroscopy and fluorescence cross-correlation spectroscopy.
This article focuses on methods based on fluctuation correlation spectroscopy to determine the formation of protein complexes in living cells. We present the principles of the fluctuation method applied to cells. We discuss the novelty and the promises of this approach. The emphasis is in the discussion of the underlying statistical assumptions of the image correlation spectroscopy analysis rat...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Biophotonics
سال: 2008
ISSN: 1864-063X,1864-0648
DOI: 10.1002/jbio.200810007